Adaptive harmonic generation microscopy of mammalian embryos
نویسندگان
چکیده
منابع مشابه
Adaptive harmonic generation microscopy of mammalian embryos.
Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live m...
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We present a detailed description of an adaptive harmonic generation (HG) microscope and culture techniques that permit long-term, three-dimensional imaging of mouse embryos. HG signal from both pre- and postimplantation stage (0.5-5.5 day-old) mouse embryos are fully characterized. The second HG images reveal central spindles during cytokinesis whereas third HG images show several features, su...
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ژورنال
عنوان ژورنال: Optics Letters
سال: 2009
ISSN: 0146-9592,1539-4794
DOI: 10.1364/ol.34.003154